Universal Soldier: Regeneration (also known in some countries as Universal Soldier: A New Beginning) is a 2009 action film being the third theatrically released film in the Universal Soldier feature film series. It is directed by John Hyams (son of director Peter Hyams, who previously worked with Jean-Claude Van Damme on two films, 1994's Timecop and 1995's Sudden Death; in this film Peter is the director of photography) with the screenplay written by Victor Ostrovsky. It stars Jean-Claude Van Damme and Dolph Lundgren, who both reprise their roles from the first film. The film is a direct sequel to the original Universal Soldier from 1992, unrelated to the two Universal Soldier television sequels that were produced in 1998 and completely ignores the events from the 1999 theatrical sequel Universal Soldier: The Return.

MMA fighter Andrei Arlovski stars as 'NGU' (Next Generation UniSol), a Universal Soldier of the latest type, along with fellow MMA fighter Mike Pyle as Capt. Kevin Burke. Tekken star Jon Foo appears as a cameo in the film as one of the four UniSols. The film was released theatrically in the Middle East and Southeast Asia and directly to video in the United States and other parts of the world. The film has received average to mixed reviews, but it has since developed a notable cult following.